Abstract
Rlyso, a highly selective and sensitive pH sensor, can stain lysosomes with a novel lysosome-locating group, methylcarbitol. Rlyso was successfully used to detect lysosomal pH changes during apoptosis or induced by chloroquine while avoiding the "alkalizing effect" on lysosomes of current lysosomal probes with nitrogen-containing sidechains.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antineoplastic Agents, Hormonal / pharmacology
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Apoptosis / drug effects
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Cell Line, Tumor
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Chloroquine / pharmacology
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Dexamethasone / pharmacology
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Ethylene Glycols / chemistry*
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Fluorescent Dyes / chemistry*
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Humans
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Hydrogen-Ion Concentration / drug effects
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Lysosomes / chemistry*
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Mice
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Microscopy, Fluorescence
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Spectrometry, Fluorescence
Substances
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Antineoplastic Agents, Hormonal
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Ethylene Glycols
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Fluorescent Dyes
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diethylene glycol monomethyl ether
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Dexamethasone
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Chloroquine