Global phylogeny of Shigella sonnei strains from limited single nucleotide polymorphisms (SNPs) and development of a rapid and cost-effective SNP-typing scheme for strain identification by high-resolution melting analysis

J Clin Microbiol. 2013 Jan;51(1):303-5. doi: 10.1128/JCM.02238-12. Epub 2012 Oct 31.

Abstract

The current Shigella sonnei pandemic involves geographically associated, multidrug-resistant clones. This study has demonstrated that S. sonnei phylogeny can be accurately defined with limited single nucleotide polymorphisms (SNPs). By typing 6 informative SNPs using a high-resolution melting (HRM) assay, major S. sonnei lineages/sublineages can be identified as defined by whole-genome variation.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Typing Techniques / methods*
  • Dysentery, Bacillary / epidemiology
  • Dysentery, Bacillary / microbiology
  • Global Health
  • Humans
  • Molecular Epidemiology / methods
  • Molecular Typing / methods*
  • Pandemics
  • Phylogeny*
  • Polymorphism, Single Nucleotide*
  • Shigella sonnei / classification*
  • Shigella sonnei / genetics*
  • Shigella sonnei / isolation & purification
  • Transition Temperature