An unusual structure of a putative T cell oncogene which allows production of similar proteins from distinct mRNAs

EMBO J. 1990 Mar;9(3):857-68. doi: 10.1002/j.1460-2075.1990.tb08183.x.

Abstract

We previously identified a putative T cell oncogene on chromosome 11 near a translocation t(11;14)(p15;q11) in a human T cell tumour. The gene is transcribed from distinct promoters which have unrelated sequences, which occur within close but distinct methylation-free islands and which allow cell specific production of mRNA. The alternative first exons each contain a protein initiation codon from which two species of protein can be made, differing by only a single amino acid. The protein sequence is highly conserved between man and mouse (98%) and the same single codon difference between alternative first exons is also conserved. This is, therefore, a new form of eukaryotic gene organization from which similar proteins can be made from distinct mRNA species.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Chromosomes, Human, Pair 11*
  • Chromosomes, Human, Pair 14
  • Codon / genetics
  • Exons
  • Gene Expression
  • Genomic Library
  • Humans
  • Molecular Sequence Data
  • Neoplasm Proteins / genetics*
  • Oligonucleotide Probes
  • Oncogenes*
  • Promoter Regions, Genetic
  • RNA, Messenger / genetics*
  • Receptors, Antigen, T-Cell / genetics*
  • Sequence Homology, Nucleic Acid
  • Transcription, Genetic
  • Transfection
  • Translocation, Genetic

Substances

  • Codon
  • Neoplasm Proteins
  • Oligonucleotide Probes
  • RNA, Messenger
  • Receptors, Antigen, T-Cell