Abstract
Shadoo (Sho) is a central nervous system glycoprotein with characteristics similar to those of the cellular prion protein PrP(C), each containing a highly conserved hydrophobic domain (HD) and an N-terminal repeat region. Whereas PrP(C) includes histidine-containing octarepeats, the Sho region N-terminal to the HD includes tandem positively charged "RGG boxes", predicted to bind RNA. Here, we demonstrate that Sho binds DNA and RNA in vitro via this arginine-rich region.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Arginine / chemistry
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GPI-Linked Proteins
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Lysine / chemistry
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Mice
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Nerve Tissue Proteins / chemistry*
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Nerve Tissue Proteins / genetics
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Nerve Tissue Proteins / metabolism*
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PrPC Proteins / chemistry
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PrPC Proteins / metabolism
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RNA, Bacterial / metabolism*
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Trinucleotide Repeats / physiology
Substances
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GPI-Linked Proteins
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Nerve Tissue Proteins
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PrPC Proteins
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RNA, Bacterial
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Sprn protein, mouse
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Arginine
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Lysine