Clicked europium dipicolinate complexes for protein X-ray structure determination

Chem Commun (Camb). 2012 Dec 18;48(97):11886-8. doi: 10.1039/c2cc36982f.

Abstract

New trisdipicolinic acid-lanthanide complexes are reported as phasing agents for X-ray crystallography of proteins. It is demonstrated that CuAAC modifications allow protein co-crystallization with low concentration of lanthanide complexes leading to an accurate structure determination.

MeSH terms

  • Crystallography, X-Ray
  • Europium / chemistry*
  • Models, Molecular
  • Molecular Structure
  • Muramidase / chemistry*
  • Muramidase / metabolism
  • Organometallic Compounds / chemical synthesis
  • Organometallic Compounds / chemistry*
  • Picolinic Acids / chemistry*
  • Plant Proteins / chemistry*

Substances

  • Organometallic Compounds
  • Picolinic Acids
  • Plant Proteins
  • Europium
  • thaumatin protein, plant
  • hen egg lysozyme
  • Muramidase