Abstract
We examined the capability of a mouse immunogenicity assay to detect improper storage of a recombinant protective antigen (rPA)-based anthrax vaccine formulated with an aluminum adjuvant, using ELISA and a toxin neutralization assay (TNA) to measure the antibody response to rPA. The vaccine was stored at 4 °C, room temperature (RT) or 37 °C for one, four and eight weeks and used for immunization, along with freshly prepared vaccine. Results showed that, contrary to ELISA, TNA is suitable to detect a loss of immunogenicity of the rPA vaccine following its exposure to RT for a period of eight weeks and to 37 °C for a period as short as 1 week.
Published by Elsevier Ltd.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adjuvants, Immunologic / chemistry
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Aluminum Hydroxide / chemistry
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Aluminum Hydroxide / immunology
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Animals
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Anthrax / immunology
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Anthrax / microbiology
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Anthrax / prevention & control
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Anthrax Vaccines / administration & dosage
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Anthrax Vaccines / chemistry
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Anthrax Vaccines / immunology*
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Antibodies, Bacterial / blood
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Antibodies, Bacterial / immunology*
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Antigens, Bacterial / genetics
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Antigens, Bacterial / immunology*
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Antigens, Bacterial / toxicity
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Bacillus anthracis / genetics
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Bacillus anthracis / immunology
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Bacterial Toxins / immunology
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Bacterial Toxins / toxicity
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Cell Line
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Cell Survival / drug effects
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Cell Survival / immunology
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Drug Storage / methods
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Enzyme-Linked Immunosorbent Assay / methods*
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Female
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Immunization
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Mice
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Neutralization Tests / methods*
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Recombinant Proteins / chemistry
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Recombinant Proteins / immunology
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Reproducibility of Results
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Temperature
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Time Factors
Substances
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Adjuvants, Immunologic
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Anthrax Vaccines
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Antibodies, Bacterial
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Antigens, Bacterial
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Bacterial Toxins
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Recombinant Proteins
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anthrax toxin
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Aluminum Hydroxide