Abstract
A stable intramolecular complex comprising the LIM domains of the LIM-homeodomain protein Isl1 tethered to a peptide region of Ldb1 has been engineered, purified and crystallized. The orthorhombic crystals belonged to space group P222(1), with unit-cell parameters a=57.2, b=56.7, c=179.8 Å, and diffracted to 3.10 Å resolution.
MeSH terms
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Animals
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Crystallization
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Crystallography, X-Ray
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DNA-Binding Proteins / chemistry*
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LIM Domain Proteins / chemistry*
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LIM-Homeodomain Proteins / chemistry*
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Mice
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Multiprotein Complexes / chemistry
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Protein Structure, Tertiary
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Transcription Factors / chemistry*
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Zinc / chemistry
Substances
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DNA-Binding Proteins
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LIM Domain Proteins
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LIM-Homeodomain Proteins
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Ldb1 protein, mouse
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Multiprotein Complexes
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Transcription Factors
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insulin gene enhancer binding protein Isl-1
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Zinc