Establishing a platform cell factory through engineering of yeast acetyl-CoA metabolism

Yun Chen; Laurent Daviet; Michel Schalk; Verena Siewers; Jens Nielsen

doi:10.1016/j.ymben.2012.11.002

Establishing a platform cell factory through engineering of yeast acetyl-CoA metabolism

Metab Eng. 2013 Jan:15:48-54. doi: 10.1016/j.ymben.2012.11.002. Epub 2012 Nov 17.

Authors

Yun Chen¹, Laurent Daviet, Michel Schalk, Verena Siewers, Jens Nielsen

Affiliation

¹ Department of Chemical and Biological Engineering, Chalmers University of Technology, SE-41296 Gothenburg, Sweden.

PMID: 23164578
DOI: 10.1016/j.ymben.2012.11.002

Abstract

Production of fuels and chemicals by industrial biotechnology requires efficient, safe and flexible cell factory platforms that can be used for production of a wide range of compounds. Here we developed a platform yeast cell factory for efficient provision of acetyl-CoA that serves as precursor metabolite for a wide range of industrially interesting products. We demonstrate that the platform cell factory can be used to improve the production of α-santalene, a plant sesquiterpene that can be used as a perfume by four-fold. This strain would be a useful tool to produce a wide range of acetyl-CoA-derived products.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acetyl Coenzyme A / genetics*
Acetyl Coenzyme A / metabolism*
Batch Cell Culture Techniques / methods*
Genetic Enhancement / methods*
Protein Engineering / methods
Saccharomyces cerevisiae / physiology*
Saccharomyces cerevisiae Proteins / physiology*
Sesquiterpenes / isolation & purification
Sesquiterpenes / metabolism*

Substances

Saccharomyces cerevisiae Proteins
Sesquiterpenes
Acetyl Coenzyme A