Abstract
Breast cancer causes death due to distant metastases in which tumor cells produce matrix metalloproteinase (MMP) enzymes which facilitate invasion. Oleuropein, the main olive oil polyphenol, has anti-proliferative effects. This study aimed to investigate the effect of oleuropein on the metastatic and anti-metastatic gene expression in the MDA human breast cancer cell line. We evaluated the MMPs and TIMPs gene expression by semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) in treated and untreated cells. This study demonstrated that OL may induce anti-metastatic effects on human breast cancer cells. We found that TIMP1,-3, and -4 were over-expressed after all periods of incubation in treated cancer cells compared to untreated cells, while MMP2 and MMP9 genes were down-regulated, at least initially. Treatment of breast cancer cells with oleuropein could help in prevention of cancer metastasis by increasing the TIMPs and suppressing the MMPs gene expressions.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Analysis of Variance
-
Antineoplastic Agents / pharmacology*
-
Breast Neoplasms / genetics*
-
Breast Neoplasms / pathology
-
Cell Line, Tumor
-
Down-Regulation / drug effects
-
Gene Expression Regulation, Neoplastic / drug effects*
-
Humans
-
Iridoid Glucosides
-
Iridoids
-
Matrix Metalloproteinase 2 / genetics
-
Matrix Metalloproteinase 9 / genetics
-
Neoplasm Metastasis
-
Pyrans / pharmacology*
-
RNA, Messenger / genetics
-
RNA, Messenger / metabolism
-
Tissue Inhibitor of Metalloproteinase-1 / genetics
-
Tissue Inhibitor of Metalloproteinase-3 / genetics
-
Tissue Inhibitor of Metalloproteinase-4
-
Tissue Inhibitor of Metalloproteinases / genetics
-
Up-Regulation / drug effects
Substances
-
Antineoplastic Agents
-
Iridoid Glucosides
-
Iridoids
-
Pyrans
-
RNA, Messenger
-
TIMP1 protein, human
-
TIMP3 protein, human
-
Tissue Inhibitor of Metalloproteinase-1
-
Tissue Inhibitor of Metalloproteinase-3
-
Tissue Inhibitor of Metalloproteinases
-
oleuropein
-
Matrix Metalloproteinase 2
-
Matrix Metalloproteinase 9