MLVA-16 loci panel on Brucella spp. using multiplex PCR and multicolor capillary electrophoresis

Giuliano Garofolo; Massimo Ancora; Elisabetta Di Giannatale

doi:10.1016/j.mimet.2012.11.007

MLVA-16 loci panel on Brucella spp. using multiplex PCR and multicolor capillary electrophoresis

J Microbiol Methods. 2013 Feb 15;92(2):103-7. doi: 10.1016/j.mimet.2012.11.007. Epub 2012 Nov 19.

Authors

Giuliano Garofolo¹, Massimo Ancora, Elisabetta Di Giannatale

Affiliation

¹ Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale - National and OIE Reference Laboratory for Brucellosis, Italy. [email protected]

PMID: 23174277
DOI: 10.1016/j.mimet.2012.11.007

Abstract

The Multi Locus Variable Number Tandem Repeat Analysis 16 loci panel (MLVA-16), involving singleplex PCRs and agarose gel electrophoresis, is the standard genotyping method for Brucella spp., used also for the Brucella international online database. We describe an alternative, reliable, high-throughput MLVA-16 protocol using multiplex PCRs and multicolor capillary electrophoresis.

MeSH terms

Brucella / classification*
Brucella / genetics*
Electrophoresis, Capillary / methods*
Humans
Minisatellite Repeats*
Molecular Typing / methods
Multiplex Polymerase Chain Reaction / methods*