miR-137 inhibits the proliferation of lung cancer cells by targeting Cdc42 and Cdk6

FEBS Lett. 2013 Jan 4;587(1):73-81. doi: 10.1016/j.febslet.2012.11.004. Epub 2012 Nov 21.

Abstract

MicroRNAs (miRNA) have emerged as key players in carcinogenesis. Here, we investigated the role of miR-137 in the pathogenesis of lung cancer. The downregulation of miR-137 in lung cancer cells could be rescued following inhibition of DNA methylation. Ectopic expression of miR-137 in lung cancer cells significantly downregulated Cdc42, Cdk6 and induced G1 cell cycle arrest, leading to a significant decrease in cell growth in vivo and in vitro. Further, both Cdc42 and Cdk6 were confirmed as targets of miR-137.

MeSH terms

  • Animals
  • Antineoplastic Agents / pharmacology
  • Carcinoma, Non-Small-Cell Lung / drug therapy
  • Carcinoma, Non-Small-Cell Lung / metabolism
  • Carcinoma, Non-Small-Cell Lung / therapy
  • Cell Line, Tumor
  • Cell Proliferation
  • Cyclin-Dependent Kinase 6 / antagonists & inhibitors*
  • Cyclin-Dependent Kinase 6 / genetics
  • Cyclin-Dependent Kinase 6 / metabolism
  • DNA Modification Methylases / antagonists & inhibitors
  • Down-Regulation*
  • G1 Phase / drug effects
  • Gene Expression Regulation, Neoplastic
  • Gene Silencing
  • Gene Transfer Techniques
  • Histone Deacetylase Inhibitors / pharmacology
  • Humans
  • Lung / drug effects
  • Lung / metabolism*
  • Lung Neoplasms / drug therapy
  • Lung Neoplasms / metabolism*
  • Lung Neoplasms / therapy
  • Mice
  • MicroRNAs / genetics
  • MicroRNAs / metabolism*
  • Xenograft Model Antitumor Assays
  • cdc42 GTP-Binding Protein / antagonists & inhibitors*
  • cdc42 GTP-Binding Protein / genetics
  • cdc42 GTP-Binding Protein / metabolism

Substances

  • Antineoplastic Agents
  • Histone Deacetylase Inhibitors
  • MIRN137 microRNA, human
  • MIRN137 microRNA, mouse
  • MicroRNAs
  • DNA Modification Methylases
  • CDK6 protein, human
  • Cdk6 protein, mouse
  • Cyclin-Dependent Kinase 6
  • cdc42 GTP-Binding Protein