Microfluidic cell sorter (μFCS) for on-chip capture and analysis of single cells

Adv Healthc Mater. 2012 Jul;1(4):432-6. doi: 10.1002/adhm.201200046. Epub 2012 May 2.

Abstract

A low-cost and rapid microfluidic cell sorter (μFCS) for circulating tumor cell (CTC) detection, culture and analyses is developed. Based on size separation and molecular characterization, μFCS efficiently enriches CTCs from unprocessed whole blood, allows on-chip culture and molecular profiling, and provides cell retrieval for subsequent analyses. The potential clinical application of the technology is demonstrated by capturing and genetically analyzing CTCs in tumor-bearing mice.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Biological Assay / instrumentation*
  • Biological Assay / methods
  • Cell Line, Tumor
  • Cell Separation / instrumentation*
  • Cell Separation / methods
  • Equipment Design
  • Equipment Failure Analysis
  • Flow Cytometry / instrumentation*
  • Flow Cytometry / methods
  • Humans
  • Microfluidic Analytical Techniques / instrumentation*
  • Microfluidic Analytical Techniques / methods
  • Neoplastic Cells, Circulating / pathology*