An in vitro Screening for Tomato Genotypes Exhibiting Efficient Shoot Regeneration

A Zelcer; O Soferman; S Izhar

doi:10.1016/S0176-1617(84)80123-6

An in vitro Screening for Tomato Genotypes Exhibiting Efficient Shoot Regeneration

J Plant Physiol. 1984 Jul;115(3):211-5. doi: 10.1016/S0176-1617(84)80123-6. Epub 2012 Feb 20.

Authors

A Zelcer¹, O Soferman, S Izhar

Affiliation

¹ Department of Plant Genetics and Breeding, Agricultural Research Organization, The Volcani Center, Bet Dagan 50-250, Israel.

PMID: 23194577
DOI: 10.1016/S0176-1617(84)80123-6

Abstract

Over 100 genotypes of tomato (Lycopersicon esculentum, wild relatives and suspected interspecific crosses) were assayed for shoot regeneration from in vitro cultured hypocotyl segments. Expiants incubated for 8 weeks in Murashige and Skoog medium supplemented with 2% glucose, 1 mg zeatin and 0.02 mg · l(-1) IAA, exhibited the best overall shoot regeneration. A wide variability in this response was observed, and approximately one-fourth (23/89) of the L. esculentum tested genotypes exhibited relatively high differentiation capabilities (>5 shoots per expiant).