Some lupane-type triterpenes inhibit tumor promotion by 12-O-tetradecanoylphorbol-13-acetate in two-stage carcinogenesis in mouse skin

K Yasukawa; S Yu; S Yamanouchi; M Takido; T Akihisa; T Tamura

doi:10.1016/S0944-7113(11)80008-5

Some lupane-type triterpenes inhibit tumor promotion by 12-O-tetradecanoylphorbol-13-acetate in two-stage carcinogenesis in mouse skin

Phytomedicine. 1995 Apr;1(4):309-13. doi: 10.1016/S0944-7113(11)80008-5.

Authors

K Yasukawa¹, S Yu, S Yamanouchi, M Takido, T Akihisa, T Tamura

Affiliation

¹ College of Pharmacy, Nihon University, 7-7-1, Narashinodai, Funabashi-shi, Chiba 274, Japan.

PMID: 23196020
DOI: 10.1016/S0944-7113(11)80008-5

Abstract

We have found that several lupane-type triterpenes, including lupeol, its acetate, betulin and betulinic acid, inhibit 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced inflammation, and that betulinic acid inhibits tumor promotion in two-stage carcinogenesis in mice. Among seven lupane-type triterpenes assayed, these compounds inhibited the inflammatory activity induced by TPA in mice. The 50 % inhibitory dose of these compounds for TPA-induced inflammation was 0.4-4.0 μmol. Furthermore, topical application of lupeol, lupeol 3-acetate and betulin markedly suppressed the tumor-promoting effect of TPA (1 μg/mouse) in mouse skin initiated with 7,12-dimethyl-benz[a]anthracene (50 μg/mouse), at a grade corresponding to that of betulinic acid.