Abstract
Hydrogels are hydrophilic polymers that have a wide range of biomedical applications including bone tissue engineering. In this study we report preparation and characterization of a thermosensitive hydrogel (Zn-CS/β-GP) containing zinc (Zn), chitosan (CS) and beta-glycerophosphate (β-GP) for bone tissue engineering. The prepared hydrogel exhibited a liquid state at room temperature and turned into a gel at body temperature. The hydrogel was characterized by SEM, EDX, XRD, FT-IR and swelling studies. The hydrogel enhanced antibacterial activity and promoted osteoblast differentiation. Thus, we suggest that the Zn-CS/β-GP hydrogel could have potential impact as an injectable in situ forming scaffold for bone tissue engineering applications.
Copyright © 2012 Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Bacterial Agents / pharmacology
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Bone and Bones / drug effects*
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Cell Death / drug effects
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Cell Differentiation / drug effects
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Chitosan / pharmacology*
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Escherichia coli / drug effects
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Glycerophosphates / pharmacology*
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Humans
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Hydrogel, Polyethylene Glycol Dimethacrylate / pharmacology*
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Hydrogen-Ion Concentration
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Injections
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Microbial Sensitivity Tests
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Microscopy, Electron, Scanning
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Osteoblasts / cytology
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Osteoblasts / drug effects
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Spectroscopy, Fourier Transform Infrared
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Staphylococcus aureus / drug effects
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Temperature*
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Time Factors
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Tissue Engineering*
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X-Ray Diffraction
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Zinc / pharmacology*
Substances
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Anti-Bacterial Agents
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Glycerophosphates
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Hydrogel, Polyethylene Glycol Dimethacrylate
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Chitosan
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Zinc
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beta-glycerophosphoric acid