Hepatic glucokinase modulates obesity predisposition by regulating BAT thermogenesis via neural signals

Cell Metab. 2012 Dec 5;16(6):825-32. doi: 10.1016/j.cmet.2012.11.006.

Abstract

Considering the explosive increase in obesity worldwide, there must be an unknown mechanism(s) promoting energy accumulation under conditions of overnutrition. We identified a feed-forward mechanism favoring energy storage, originating in hepatic glucokinase (GK) upregulation. High-fat feeding induced hepatic GK upregulation, and hepatic GK overexpression dose-dependently decreased adaptive thermogenesis by downregulating thermogenesis-related genes in brown adipose tissue (BAT). This intertissue (liver-to-BAT) system consists of the afferent vagus from the liver and sympathetic efferents from the medulla and antagonizes anti-obesity effects of leptin on thermogenesis. Furthermore, upregulation of endogenous GK in the liver by high-fat feeding was more marked in obesity-prone than in obesity-resistant strains and was inversely associated with BAT thermogenesis. Hepatic GK overexpression in obesity-resistant mice promoted weight gain, while hepatic GK knockdown in obesity-prone mice attenuated weight gain with increased adaptive thermogenesis. Thus, this intertissue energy-saving system may contribute to determining obesity predisposition.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipose Tissue, Brown / metabolism*
  • Animals
  • Diet, High-Fat
  • Glucokinase / antagonists & inhibitors
  • Glucokinase / genetics
  • Glucokinase / metabolism*
  • Glycogen / metabolism
  • Leptin / metabolism
  • Liver / enzymology*
  • Mice
  • Mice, Inbred C57BL
  • Neurons / metabolism*
  • Obesity / metabolism*
  • Obesity / pathology
  • RNA Interference
  • RNA, Small Interfering / metabolism
  • Signal Transduction
  • Thermogenesis / genetics*
  • Up-Regulation
  • Weight Gain

Substances

  • Leptin
  • RNA, Small Interfering
  • Glycogen
  • Glucokinase