Cryotherapy reduces skeletal muscle damage after ischemia/reperfusion in rats

J Anat. 2013 Feb;222(2):223-30. doi: 10.1111/joa.12009. Epub 2012 Dec 11.

Abstract

The aim of this study was to analyze the effects of cryotherapy on the biochemical and morphological changes in ischemic and reperfused (I/R) gastrocnemius muscle of rats. Forty male Wistar rats were divided into control and I/R groups, and divided based on whether or not the rats were submitted to cryotherapy. Following the reperfusion period, biochemical and morphological analyses were performed. Following cryotherapy, a reduction in thiobarbituric acid-reactive substances and dichlorofluorescein oxidation levels were observed in I/R muscle. Cryotherapy in I/R muscle also minimized effects such as decreased cellular viability, levels of non-protein thiols and calcium ATPase activity as well as increased catalase activity. Cryotherapy also limited mitochondrial dysfunction and decreased the presence of neutrophils in I/R muscle, an effect that was corroborated by reduced myeloperoxidase activity in I/R muscle treated with cryotherapy. The effects of cryotherapy are associated with a reduction in the intensity of the inflammatory response and also with a decrease in mitochondrial dysfunction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Analysis of Variance
  • Animals
  • Biomarkers / metabolism
  • Cell Survival / physiology
  • Cryotherapy*
  • Disease Models, Animal
  • Ischemia / enzymology
  • Ischemia / physiopathology
  • Ischemia / therapy*
  • Male
  • Mitochondria, Muscle / enzymology
  • Muscle, Skeletal / blood supply*
  • Muscle, Skeletal / enzymology
  • Oxidative Stress / physiology
  • Peroxidase / metabolism
  • Rats
  • Rats, Wistar
  • Reperfusion Injury / enzymology
  • Reperfusion Injury / physiopathology
  • Reperfusion Injury / therapy*

Substances

  • Biomarkers
  • Peroxidase