Abstract
Enterovirus 70 (EV70) is the causative agent of acute hemorrhagic conjunctivitis (AHC), for which no effective vaccine is available. This study revealed a high reactivity of the N-terminal region of EV70 VP1 (VP1-1) with an anti-EV70 mouse serum. The analysis of overlapping synthetic peptides of VP1-1 identified a B-cell epitope in this region. The E-peptide (14-ANTVESEIKAELGVI-28) showing the highest reactivity with the anti-EV70 serum induced neutralizing antibodies in mice and reduced the virus titer in the eyes, suggesting that it is a candidate vaccine against AHC caused by EV70.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Viral / immunology
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Antibody Formation
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Capsid Proteins / genetics
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Capsid Proteins / immunology*
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Conjunctivitis, Acute Hemorrhagic / immunology*
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Conjunctivitis, Acute Hemorrhagic / prevention & control
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Conjunctivitis, Acute Hemorrhagic / virology
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Enterovirus D, Human / genetics
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Enterovirus D, Human / immunology*
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Epitopes, B-Lymphocyte / immunology*
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Humans
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Mice
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Mice, Inbred BALB C
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Peptides / genetics
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Peptides / immunology*
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Vaccines, Subunit / immunology*
Substances
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Antibodies, Viral
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Capsid Proteins
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Epitopes, B-Lymphocyte
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Peptides
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Vaccines, Subunit