Abstract
Fluorescent dyes that are bright, stable, small, and biocompatible are needed for high-sensitivity two-photon imaging, but the combination of these traits has been elusive. We identified a class of squaraine derivatives with large two-photon action cross-sections (up to 10,000 GM) at near-infrared wavelengths critical for in vivo imaging. We demonstrate the biocompatibility and stability of a red-emitting squaraine-rotaxane (SeTau-647) by imaging dye-filled neurons in vivo over 5 days, and utility for sensitive subcellular imaging by synthesizing a specific peptide-conjugate label for the synaptic protein PSD-95.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cyclobutanes / chemistry*
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Cyclobutanes / metabolism
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Fluorescent Dyes / chemistry*
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Fluorescent Dyes / metabolism
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Microscopy, Fluorescence, Multiphoton / methods*
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Nerve Tissue Proteins / chemistry
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Nerve Tissue Proteins / metabolism
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Neurons / chemistry
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Neurons / metabolism
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Phenols / chemistry*
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Phenols / metabolism
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Photons
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Rotaxanes / chemistry
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Rotaxanes / metabolism
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SAP90-PSD95 Associated Proteins
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Synapses / chemistry
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Synapses / metabolism
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Xenopus laevis
Substances
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Cyclobutanes
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Fluorescent Dyes
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Nerve Tissue Proteins
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Phenols
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Rotaxanes
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SAP90-PSD95 Associated Proteins
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squaraine