Identification of mitogen-responsive ribosomal protein S6 kinase pp90rsk, a homolog of Xenopus S6 kinase II, in chicken embryo fibroblasts

Mol Cell Biol. 1990 May;10(5):2413-7. doi: 10.1128/mcb.10.5.2413-2417.1990.

Abstract

Antiserum raised against recombinant Xenopus ribosomal protein S6 kinase (rsk) was used to identify a 90,000-Mr ribosomal S6 kinase, pp90rsk, in chicken embryo fibroblasts. Adding serum to cells stimulated the phosphorylation of pp90rsk on serine and threonine residues and increased the activity of S6 kinase measured in immune complex assays. Xenopus S6 kinase II and chicken embryo fibroblast pp90rsk had nearly identical phosphopeptide maps.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Blotting, Western
  • Chick Embryo
  • Molecular Weight
  • Peptide Mapping
  • Phosphoproteins / analysis
  • Phosphoproteins / metabolism
  • Precipitin Tests
  • Protein Kinases / analysis*
  • Ribosomal Protein S6 Kinases

Substances

  • Phosphoproteins
  • Protein Kinases
  • Ribosomal Protein S6 Kinases