The separation and quantification of glycosaminoglycan (GAG) chains with different levels of sulfation from cells and media, and prepared through chemoenzymatic synthesis or metabolic engineering, pose a major challenge in glycomics analysis. A method for microscale separation and quantification of heparin, heparan sulfate, and heparosan from cells is reported. This separation relies on a mini strong anion exchange spin column eluted stepwise with various concentrations of sodium chloride. Disaccharide analysis by LC-MS was used to monitor the chemical structure of the various GAG chains that were recovered.
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