Highly enantioselective oxidation of α-hydroxyacids bearing a substituent with an aryl group: co-production of optically active α-hydroxyacids and α-ketoacids

Bioresour Technol. 2013 Mar:132:391-4. doi: 10.1016/j.biortech.2012.11.067. Epub 2012 Nov 29.

Abstract

A novel and simple methodology for co-obtaining of enantiomerically pure α-hydroxyacids and α-ketoacids was developed by enantioselective oxidation of α-hydroxyacids bearing a substituent with an aryl group using α-hydroxyacid dehydrogenase (α-HADH). A high-throughput method was firstly established for screening of enantioselective α-HADHs. Sinorhizobium sp. ZJB1 101 with high activity and excellent enantioselectivity of α-HADH for oxidation of α-hydroxyacids bearing a substituent with an aryl group was isolated and identified. This strain has potential for co-production of (R)-α-hydroxyacids and α-ketoacids in near theoretical yields, while no consecutive oxidation of α-ketoacids was observed. The green conversion system appears promising for potential applications in industry.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alcohol Oxidoreductases / chemistry
  • Alcohol Oxidoreductases / metabolism*
  • Biotechnology / methods*
  • Catalysis
  • High-Throughput Screening Assays / methods*
  • Hydroxy Acids / chemistry
  • Hydroxy Acids / isolation & purification*
  • Hydroxy Acids / metabolism
  • Keto Acids / chemistry
  • Keto Acids / isolation & purification*
  • Keto Acids / metabolism
  • Molecular Structure
  • Oxidation-Reduction
  • Sinorhizobium / enzymology*

Substances

  • Hydroxy Acids
  • Keto Acids
  • Alcohol Oxidoreductases
  • D-2-hydroxyacid dehydrogenase