Epidemiology of pneumococcal serotype 6A and 6C among invasive and carriage isolates from Alaska, 1986-2009

Diagn Microbiol Infect Dis. 2013 Mar;75(3):271-6. doi: 10.1016/j.diagmicrobio.2012.11.021. Epub 2012 Dec 28.

Abstract

We investigated serotype 6A/6C invasive pneumococcal disease (IPD) incidence, genetic diversity, and carriage before and after 7-valent pneumococcal conjugate vaccine (PCV7) introduction in Alaska. IPD cases (1986-2009) were identified through population-based laboratory surveillance. Isolates were initially serotyped by conventional methods, and 6C isolates were differentiated from 6A by polymerase chain reaction. Among invasive and carriage isolates initially typed as 6A, 35% and 50% were identified as 6C, respectively. IPD rates caused by serotype 6A or 6C among children <5 years did not change from the pre- to post-PCV7 period (P = 0.71 and P = 0.09, respectively). Multilocus sequence typing of IPD isolates revealed 28 sequence types. The proportion of serotype 6A carriage isolates decreased from 7.4% pre-PCV7 to 1.8% (P < 0.001) during 2008-2009; the proportion of serotype 6C carriage isolates increased from 3.0% to 8.4% (P = 0.004) among children <5 years. Continued surveillance is warranted to monitor changes in serotype distribution and prevalence.

MeSH terms

  • Alaska / epidemiology
  • Anti-Bacterial Agents / pharmacology
  • Carrier State / microbiology*
  • Child
  • Child, Preschool
  • Genetic Variation
  • Genotype
  • Heptavalent Pneumococcal Conjugate Vaccine
  • Humans
  • Incidence
  • Microbial Sensitivity Tests
  • Multilocus Sequence Typing
  • Penicillins / pharmacology
  • Pneumococcal Infections / epidemiology*
  • Pneumococcal Infections / microbiology
  • Pneumococcal Infections / prevention & control
  • Pneumococcal Vaccines / administration & dosage
  • Rural Population
  • Streptococcus pneumoniae / classification
  • Streptococcus pneumoniae / drug effects
  • Streptococcus pneumoniae / genetics
  • Streptococcus pneumoniae / isolation & purification*

Substances

  • Anti-Bacterial Agents
  • Heptavalent Pneumococcal Conjugate Vaccine
  • Penicillins
  • Pneumococcal Vaccines