Abstract
A bacteriocin-producing (11,000 AU mL(-1)) strain was isolated from the rhizosphere of healthy Algerian plants Ononis angustissima Lam., and identified as Brevibacillus brevis strain GM100. The bacteriocin, called Bac-GM100, was purified to homogeneity from the culture supernatant, and, based on MALDI-TOF/MS analysis, was a monomer protein with a molecular mass of 4375.66 Da. The 21 N-terminal residues of Bac-GM100 displayed 65% homology with thurincin H from Bacillus thuringiensis. Bac-GM100 was extremely heat-stable (20 min at 120 °C), and was stable within a pH range of 3-10. It proved sensitive to various proteases, which demonstrated its protein nature. It was also found to display a bactericidal mode of action against gram-negative (Salmonella enteric ATCC 43972, Pseudomonas aeruginosa ATCC 49189, and Agrobacterium tumefaciens C58) and gram-positive (Enterococcus faecalis ENSAIA 631 and Staphylococcus aureus ATCC 6538) bacteria, and a fungistatic mode of action against the pathogenic fungus Candida tropicalis R2 CIP 203.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Algeria
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Anti-Infective Agents / isolation & purification*
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Anti-Infective Agents / metabolism*
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Anti-Infective Agents / pharmacology
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Bacillus thuringiensis / chemistry
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Bacterial Proteins / isolation & purification*
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Bacterial Proteins / metabolism*
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Bacterial Proteins / pharmacology
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Bacteriocins / chemistry
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Bacteriocins / isolation & purification*
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Bacteriocins / metabolism*
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Bacteriocins / pharmacology
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Brevibacillus / chemistry*
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Brevibacillus / genetics
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Brevibacillus / isolation & purification
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Candida tropicalis / drug effects
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Candida tropicalis / growth & development
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Enterococcus faecalis / drug effects
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Enterococcus faecalis / growth & development
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Fabaceae / microbiology
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Hot Temperature
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Hydrogen-Ion Concentration
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Microbial Viability / drug effects
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Molecular Weight
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Phylogeny
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Protein Stability
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Pseudomonas aeruginosa / drug effects
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Pseudomonas aeruginosa / growth & development
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Rhizosphere
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Salmonella enterica / drug effects
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Salmonella enterica / growth & development
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Staphylococcus aureus / drug effects
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Staphylococcus aureus / growth & development
Substances
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Anti-Infective Agents
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Bacterial Proteins
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Bacteriocins
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thurincin H