Structural analysis of sulfated fucan from Saccharina japonica by electrospray ionization tandem mass spectrometry

Weihua Jin; Zhimou Guo; Jing Wang; Wenjing Zhang; Quanbin Zhang

doi:10.1016/j.carres.2012.12.004

Structural analysis of sulfated fucan from Saccharina japonica by electrospray ionization tandem mass spectrometry

Carbohydr Res. 2013 Mar 22:369:63-7. doi: 10.1016/j.carres.2012.12.004. Epub 2012 Dec 12.

Authors

Weihua Jin¹, Zhimou Guo, Jing Wang, Wenjing Zhang, Quanbin Zhang

Affiliation

¹ Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, PR China.

PMID: 23298554
DOI: 10.1016/j.carres.2012.12.004

Abstract

Desulfation of a fucoidan from Saccharina japonica by treatment with DMSO-MeOH resulted in partial degradation of polymeric molecules by methanolysis giving rise to a mixture of neutral, monosulfated, and disulfated fucooligosaccharides in the form of methyl glycosides. These oligomeric fragments were characterized by ESI-MS and ESI-CID-MS/MS. It was found that oligosaccharide structures coincided with the polysaccharide backbone built up mainly of (1→3)-linked fucose residues sulfated at positions 4 and 2.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Phaeophyceae / chemistry*
Polysaccharides / chemistry*
Spectrometry, Mass, Electrospray Ionization / methods*

Substances

Polysaccharides
fucoidan