The first Korean case of mucopolysaccharidosis IIIC (Sanfilippo syndrome type C) confirmed by biochemical and molecular investigation

Ann Lab Med. 2013 Jan;33(1):75-9. doi: 10.3343/alm.2013.33.1.75. Epub 2012 Dec 17.

Abstract

Mucopolysaccharidosis (MPS) III has 4 enzymatically distinct forms (A, B, C, and D), and MPS IIIC, also known as Sanfilippo C syndrome, is an autosomal recessive lysosomal storage disease caused by a deficiency of heparan acetyl-CoA:alpha-glucosaminide N-acetyltransferase (HGSNAT). Here, we report a case of MPS IIIC that was confirmed by molecular genetic analysis. The patient was a 2-yr-old girl presenting with skeletal deformity, hepatomegaly, and delayed motor development. Urinary excretion of glycosaminoglycan (GAG) was markedly elevated (984.4 mg GAG/g creatinine) compared with the age-specific reference range (<175 mg GAG/g creatinine), and a strong band of heparan sulfate was recognized on performing thin layer chromatography. HGSNAT enzyme activity in leukocytes was 0.7 nmol/17 hr/mg protein, which was significantly lower than the reference range (8.6-32 nmol/17 hr/mg protein). PCR and direct sequencing of the HGSNAT gene showed 2 mutations: c.234+1G>A (IVS2+1G>A) and c.1150C>T (p.Arg384*). To the best of our knowledge, this is the first case of MPS IIIC to be confirmed by clinical, biochemical, and molecular genetic findings in Korea.

Keywords: HGSNAT; Korea; Mucopolysaccharidosis IIIC; Sanfilippo syndrome.

Publication types

  • Case Reports

MeSH terms

  • Acetyltransferases / genetics*
  • Asian People / genetics*
  • Base Sequence
  • Child, Preschool
  • Chromatography, Thin Layer
  • Female
  • Glycosaminoglycans / urine
  • Heparitin Sulfate / chemistry
  • Heparitin Sulfate / metabolism
  • Humans
  • Leukocytes / immunology
  • Leukocytes / metabolism
  • Mucopolysaccharidosis III / diagnosis*
  • Mucopolysaccharidosis III / diagnostic imaging
  • Mucopolysaccharidosis III / genetics
  • Mutation
  • Radiography
  • Republic of Korea
  • Sequence Analysis, DNA

Substances

  • Glycosaminoglycans
  • Heparitin Sulfate
  • Acetyltransferases
  • HGSNAT protein, human