Effects of total glucosides of paeony for delaying onset of Sjogren's syndrome: an animal study

Chun Lei Li; Jing He; Zhan Guo Li; Li Wu Zheng; Hong Hua

doi:10.1016/j.jcms.2012.11.042

Effects of total glucosides of paeony for delaying onset of Sjogren's syndrome: an animal study

J Craniomaxillofac Surg. 2013 Oct;41(7):610-5. doi: 10.1016/j.jcms.2012.11.042. Epub 2013 Jan 18.

Authors

Chun Lei Li¹, Jing He, Zhan Guo Li, Li Wu Zheng, Hong Hua

Affiliation

¹ Department of Oral Medicine and Traditional Chinese Medicine, Peking University School and Hospital of Stomatology, Beijing, China.

PMID: 23333492
DOI: 10.1016/j.jcms.2012.11.042

Abstract

Objective: To investigate the effectiveness of total glucosides of paeony (TGP) on Sjogren's syndrome (SS) using non-obese diabetic (NOD) mice model.

Study design: Twenty-seven 8-week-old female NOD mice were assigned into TGP group, hydroxychloroquine (HCQ) group and normal saline (NS) group, receiving corresponding drugs respectively and sacrificed at 24-week-old. Saliva flow rate (SFR), ration of regulatory T cells, level of anti-SSA/SSB, histological changes in submandibular glands (SMG) and microarray analysis were assessed. The data were analyzed using SPSS.

Results: Compared to NS group, in TGP group, SFR, SMG index and the ration of regulatory T cells were significantly higher, while anti-SSA/SSB and lymphocytic foci were significantly lower. HCQ group demonstrated similar results except SMG index. Altered gene expression was found in 10.71% of TGP and 13.09% of HCQ of the profile.

Conclusion: TGP demonstrated a similar effectiveness as HCQ in delaying the onset of SS-like disease in NOD mice.

Keywords: Animal model; Sialoadenitis; Sjogren's syndrome; Total glucosides of paeony.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Autoantigens / analysis
Disease Models, Animal
Enzyme Inhibitors / therapeutic use
Female
Gene Expression Profiling
Glucosides / therapeutic use*
Hydroxychloroquine / therapeutic use
Lymphocytes / drug effects
Lymphocytes / pathology
Mice
Mice, Inbred NOD
Microarray Analysis
Paeonia / chemistry*
Phytotherapy / methods*
Ribonucleoproteins / analysis
SS-B Antigen
Saliva / drug effects
Saliva / metabolism
Secretory Rate / drug effects
Sjogren's Syndrome / prevention & control*
Submandibular Gland / drug effects
Submandibular Gland / pathology
T-Lymphocytes, Regulatory / drug effects
T-Lymphocytes, Regulatory / pathology

Substances

Autoantigens
Enzyme Inhibitors
Glucosides
Ribonucleoproteins
SS-A antigen
Hydroxychloroquine