The production of 1,3-propanediol from mixtures of glycerol and glucose by a Klebsiella pneumoniae mutant deficient in carbon catabolite repression

Bioresour Technol. 2013 Feb:130:719-24. doi: 10.1016/j.biortech.2012.12.076. Epub 2012 Dec 20.

Abstract

In the present study, mutant strain of Klebsiella pneumoniae with deletion of the crr gene encoding EIIA(Glc) (a component of the glucose-specific phosphoenolpyruvate-dependent transferase system [PTS]) was prepared. This eliminated the ability of the strain to mediate carbon catabolite repression (CCR). Production of 1,3-propanediol (1,3-PD) from glycerol by the crr mutant strain was enhanced (compared to that of the parent) in the presence of glucose. Using molasses as a co-substrate of glycerol, the maximum yield of 1,3-PD was 60.4% greater (81.2g/l) than that obtained when glycerol was used alone, under optimum fermentation conditions.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / genetics
  • Catabolite Repression / genetics*
  • Fermentation
  • Glucose / metabolism*
  • Glycerol / metabolism*
  • Klebsiella pneumoniae / genetics
  • Klebsiella pneumoniae / metabolism*
  • Molasses
  • Mutation
  • Phosphoenolpyruvate Sugar Phosphotransferase System / genetics
  • Propylene Glycols / metabolism*

Substances

  • Bacterial Proteins
  • Propylene Glycols
  • 1,3-propanediol
  • Phosphoenolpyruvate Sugar Phosphotransferase System
  • Glucose
  • Glycerol