Highly efficient ionization of phosphopeptides at low pH by desorption electrospray ionization mass spectrometry

Ning Pan; Pengyuan Liu; Weidong Cui; Bo Tang; Jingmin Shi; Hao Chen

doi:10.1039/c3an36737a

Highly efficient ionization of phosphopeptides at low pH by desorption electrospray ionization mass spectrometry

Analyst. 2013 Mar 7;138(5):1321-1324. doi: 10.1039/c3an36737a.

Authors

Ning Pan^#^{1

2}, Pengyuan Liu^#², Weidong Cui³, Bo Tang¹, Jingmin Shi¹, Hao Chen²

Affiliations

¹ College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan, China, 250014.
² Center for Intelligent Chemical Instrumentation, Department of Chemistry and Biochemistry, Ohio University, Athens, OH, USA 45701.
³ Department of Chemistry, Washington University, St. Louis, MO, USA 63130.

^# Contributed equally.

Abstract

A fast and novel strategy for efficient ionization of phosphopeptides in mixtures is reported, in which the sample is acidified to low pH to suppress the deprotonation of phosphate groups and then followed by direct analysis using liquid sample desorption electrospray ionization mass spectrometry (DESI-MS).

Publication types

Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Amino Acid Sequence
Hydrogen-Ion Concentration
Molecular Sequence Data
Phosphopeptides / chemistry*
Spectrometry, Mass, Electrospray Ionization / economics
Spectrometry, Mass, Electrospray Ionization / methods*

Substances

Phosphopeptides

Abstract

Publication types

MeSH terms

Substances

Grants and funding