Total flavonoids of Scutellaria barbata inhibit invasion of hepatocarcinoma via MMP/TIMP in vitro

Zhi-Jun Dai; Bao-Feng Wang; Wang-Feng Lu; Zhi-Dong Wang; Xiao-Bin Ma; Wei-Li Min; Hua-Feng Kang; Xi-Jing Wang; Wen-Ying Wu

doi:10.3390/molecules18010934

Total flavonoids of Scutellaria barbata inhibit invasion of hepatocarcinoma via MMP/TIMP in vitro

Molecules. 2013 Jan 11;18(1):934-50. doi: 10.3390/molecules18010934.

Authors

Zhi-Jun Dai¹, Bao-Feng Wang, Wang-Feng Lu, Zhi-Dong Wang, Xiao-Bin Ma, Wei-Li Min, Hua-Feng Kang, Xi-Jing Wang, Wen-Ying Wu

Affiliation

¹ Department of Oncology, the Second Affiliated Hospital, Medical School of Xi'an Jiaotong University, Xi'an 710004, China. [email protected]

Abstract

Metastasis is the major cause of cancer-related deaths. Targeting the process of metastasis has been proposed as a strategy to fight cancer. Scutellaria barbata D. Don (S. barbata), a traditional Chinese medicine, is used for treatment of many diseases, including cancer. This study aimed to determine the anti-metastatic effect of total flavonoids of S. barbata (TF-SB) using the human hepatocarcinoma MHCC97H cell line with high metastatic potential. Our results show that TF-SB could significantly inhibit the proliferation and invasion of MHCC97H cells in a dose-dependent manner. MMP-2 and MMP-9 expression were obviously decreased after TF-SB treatment at both the mRNA and protein level. TIMP-1 and TIMP-2 expression were simultaneously increased. The present study indicates that TF-SB could reduce the metastatic capability of MHCC97H cell, probably through decrease of the MMP expression, and simultaneous increase of the TIMP expression.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antineoplastic Agents, Phytogenic / pharmacology*
Carcinoma, Hepatocellular
Cell Line, Tumor
Cell Movement / drug effects*
Cell Proliferation / drug effects
Flavonoids / pharmacology*
Gene Expression / drug effects*
Gene Expression Regulation / drug effects
Humans
Inhibitory Concentration 50
Matrix Metalloproteinase 2 / genetics
Matrix Metalloproteinase 2 / metabolism
Matrix Metalloproteinase 9 / genetics
Matrix Metalloproteinase 9 / metabolism
Plant Extracts / pharmacology*
Scutellaria / chemistry*
Tissue Inhibitor of Metalloproteinase-1 / genetics
Tissue Inhibitor of Metalloproteinase-1 / metabolism
Tissue Inhibitor of Metalloproteinase-2 / genetics
Tissue Inhibitor of Metalloproteinase-2 / metabolism

Substances

Antineoplastic Agents, Phytogenic
Flavonoids
Plant Extracts
TIMP1 protein, human
TIMP2 protein, human
Tissue Inhibitor of Metalloproteinase-1
Tissue Inhibitor of Metalloproteinase-2
MMP2 protein, human
Matrix Metalloproteinase 2
MMP9 protein, human
Matrix Metalloproteinase 9