Investigations of protein-protein interactions using time-resolved fluorescence and phasors

David M Jameson; Carissa M Vetromile; Nicholas G James

doi:10.1016/j.ymeth.2013.01.004

Investigations of protein-protein interactions using time-resolved fluorescence and phasors

Methods. 2013 Mar;59(3):278-86. doi: 10.1016/j.ymeth.2013.01.004. Epub 2013 Jan 22.

Authors

David M Jameson¹, Carissa M Vetromile, Nicholas G James

Affiliation

¹ University of Hawaii, Department of Cell and Molecular Biology, John A. Burns School of Medicine, 651 Ilalo St., BSB222, Honolulu, HI 96813, USA. [email protected]

PMID: 23348372
DOI: 10.1016/j.ymeth.2013.01.004

Abstract

Protein interactions are critical for biological specificity and techniques able to characterize these interactions are of fundamental importance in biochemistry and cell biology. Fluorescence methodologies have been extremely useful for studying many biological systems including protein-ligand and protein-protein interactions. In this review we focus on the application of time-resolved fluorescence approaches to macromolecular systems. We also include a detailed discussion of a relatively new time-resolved technique, the phasor method, for studying protein interactions both in vitro and in live cells.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Fluorescence Polarization
Fluorescence Resonance Energy Transfer / methods
Image Processing, Computer-Assisted
Microscopy, Fluorescence / methods*
Protein Interaction Mapping / methods*
Proteins / metabolism*
Software
Time Factors

Substances

Proteins