Conventional and monocyte-derived CD11b(+) dendritic cells initiate and maintain T helper 2 cell-mediated immunity to house dust mite allergen

Maud Plantinga; Martin Guilliams; Manon Vanheerswynghels; Kim Deswarte; Filipe Branco-Madeira; Wendy Toussaint; Leen Vanhoutte; Katrijn Neyt; Nigel Killeen; Bernard Malissen; Hamida Hammad; Bart N Lambrecht

doi:10.1016/j.immuni.2012.10.016

Conventional and monocyte-derived CD11b(+) dendritic cells initiate and maintain T helper 2 cell-mediated immunity to house dust mite allergen

Immunity. 2013 Feb 21;38(2):322-35. doi: 10.1016/j.immuni.2012.10.016. Epub 2013 Jan 24.

Authors

Maud Plantinga¹, Martin Guilliams, Manon Vanheerswynghels, Kim Deswarte, Filipe Branco-Madeira, Wendy Toussaint, Leen Vanhoutte, Katrijn Neyt, Nigel Killeen, Bernard Malissen, Hamida Hammad, Bart N Lambrecht

Affiliation

¹ Laboratory of Immunoregulation and Mucosal Immunology, Department for Molecular Biomedical Research, VIB, Ghent 9050, Belgium.

PMID: 23352232
DOI: 10.1016/j.immuni.2012.10.016

Abstract

Dendritic cells (DCs) are crucial for mounting allergic airway inflammation, but it is unclear which subset of DCs performs this task. By using CD64 and MAR-1 staining, we reliably separated CD11b(+) monocyte-derived DCs (moDCs) from conventional DCs (cDCs) and studied antigen uptake, migration, and presentation assays of lung and lymph node (LN) DCs in response to inhaled house dust mite (HDM). Mainly CD11b(+) cDCs but not CD103(+) cDCs induced T helper 2 (Th2) cell immunity in HDM-specific T cells in vitro and asthma in vivo. Studies in Flt3l(-/-) mice, lacking all cDCs, revealed that moDCs were also sufficient to induce Th2 cell-mediated immunity but only when high-dose HDM was given. The main function of moDCs was the production of proinflammatory chemokines and allergen presentation in the lung during challenge. Thus, we have identified migratory CD11b(+) cDCs as the principal subset inducing Th2 cell-mediated immunity in the LN, whereas moDCs orchestrate allergic inflammation in the lung.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Administration, Inhalation
Adoptive Transfer
Allergens / immunology*
Allergens / isolation & purification
Animals
Antigens, Dermatophagoides / administration & dosage
Antigens, Dermatophagoides / immunology*
Antigens, Dermatophagoides / isolation & purification
Antigens, Ly / genetics
Antigens, Ly / immunology
Asthma / immunology*
Asthma / pathology
CD11b Antigen / genetics
CD11b Antigen / immunology
Cell Movement
Cell Proliferation
Dendritic Cells / immunology*
Dendritic Cells / transplantation
Gene Expression
Immunity, Cellular*
Inflammation / immunology
Inflammation / pathology
Lung / immunology
Lung / pathology
Lymph Nodes / immunology
Lymph Nodes / pathology
Mice
Mice, Transgenic
Monocytes / immunology
Monocytes / transplantation
Natural Cytotoxicity Triggering Receptor 1 / genetics
Natural Cytotoxicity Triggering Receptor 1 / immunology
Organ Specificity
Pyroglyphidae / immunology*
Receptors, IgG / genetics
Receptors, IgG / immunology
Th2 Cells / immunology*

Substances

Allergens
Antigens, Dermatophagoides
Antigens, Ly
CD11b Antigen
Natural Cytotoxicity Triggering Receptor 1
Ncr1 protein, mouse
Receptors, IgG