Abstract
Deficiency in the PTS2 protein import pathway due to mutations in PEX7 gene results in the rhizomelic chondrodysplasia punctata (RCDP) type 1. In the present study, we have reported a novel missense mutation, W75R, in the PEX7 gene in an Iranian patient with the RCDP type 1. The inability of PEX7 protein to transport PTS2 containing proteins including peroxisomal 3-ketoacyl-CoA thiolase and PTS2-EGFP protein to the surface of the peroxisomes showed that the W75R mutation in PEX7 gene severely impaired the function of PEX7 protein and was responsible for RCDP type 1 in this patient.
Copyright © 2013 Elsevier B.V. All rights reserved.
MeSH terms
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Acetyl-CoA C-Acyltransferase / metabolism
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Amino Acid Sequence
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Base Sequence
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Cells, Cultured
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Child, Preschool
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Chondrodysplasia Punctata, Rhizomelic / genetics*
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Humans
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Iran
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Male
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Molecular Sequence Data
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Mutation, Missense*
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Peroxisomal Targeting Signal 2 Receptor
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Protein Transport / genetics
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Receptors, Cytoplasmic and Nuclear / chemistry
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Receptors, Cytoplasmic and Nuclear / genetics*
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Sequence Alignment
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Sequence Analysis, DNA
Substances
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PEX7 protein, human
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Peroxisomal Targeting Signal 2 Receptor
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Receptors, Cytoplasmic and Nuclear
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Acetyl-CoA C-Acyltransferase
Supplementary concepts
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Rhizomelic chondrodysplasia punctata, type 1