Abstract
In the present study, we developed an efficient method of 1,3-propanediol (1,3-PD) production from glycerol by genetic engineering of Klebsiella pneumoniae AK mutant strains. The proposed approach eliminated by-product formation and IPTG induction resulted in maximal production of 1,3-PD. A series of recombinant strains was designed to constitutively express the dhaB and/or dhaT genes, using the bacteriophage T5 P(DE20) promoter and the rho-independent transcription termination signal of the Rahnella aquatilis levansucrase gene. Among these strains, AK/pConT expressing dhaT alone gave the highest yield of 1,3-PD. Fed-batch fermentation resulted in efficient production of 1,3-PD from either pure or crude glycerol, without by-product formation.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Alcohol Dehydrogenase / biosynthesis*
-
Alcohol Dehydrogenase / genetics
-
Bacterial Proteins / biosynthesis*
-
Bacterial Proteins / genetics
-
Bacteriophages / genetics
-
Bacteriophages / metabolism
-
Cryoprotective Agents / metabolism
-
Cryoprotective Agents / pharmacology
-
Gene Expression*
-
Glycerol / metabolism*
-
Glycerol / pharmacology
-
Hexosyltransferases / biosynthesis
-
Hexosyltransferases / genetics
-
Klebsiella pneumoniae / genetics
-
Klebsiella pneumoniae / metabolism*
-
Metabolic Engineering*
-
Promoter Regions, Genetic
-
Propylene Glycols / metabolism*
-
Rahnella / enzymology
-
Rahnella / genetics
-
Viral Proteins / biosynthesis
-
Viral Proteins / genetics
Substances
-
Bacterial Proteins
-
Cryoprotective Agents
-
Propylene Glycols
-
Viral Proteins
-
1,3-propanediol
-
Alcohol Dehydrogenase
-
1,3-propanediol dehydrogenase
-
Hexosyltransferases
-
levansucrase
-
Glycerol