An increasing number of new types of human enteroviruses (HEV) have been identified with the application of molecular typing method based on VP1 sequence analysis. In this study, the non-polio enteroviruses (NPEV) isolated from acute flaccid paralysis (AFP) cases in Shandong Province, China were typed via molecular typing method, and 1 EV74, 3 EV80 and 1 EV87 strains were identified. Homologous comparison revealed EV74, EV80 and EV87 Shandong strains had 81.4%, 76.4%-81.7%, and 80.3% VP1 identities with prototype strains. Phylogenetic analysis suggested a remote genetic distance with other strains. This is the first report of EV74 and EV87 in mainland China, and the low isolation rate in AFP surveillance suggested these three serotypes has not been the predominant viruses in China.