Abstract
The authors describe a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method targeting the 18S rRNA gene for the species detection of medically important trematode infecting fish and oysters, and suggest that this PCR-RFLP method based on a specific Tre-18 primer and the restriction enzymes, Acc1, Ava2, Msp1, and Hinf1, is useful for the detection of parasites in aquatic food.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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DNA Primers / genetics
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DNA Restriction Enzymes / genetics
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DNA, Helminth / isolation & purification*
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Fishes / parasitology
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Food Contamination / analysis
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Food Parasitology
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Helminths / classification
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Helminths / isolation & purification
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Helminths / pathogenicity
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Ostreidae / parasitology
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Polymerase Chain Reaction / methods*
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Polymorphism, Restriction Fragment Length*
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RNA, Ribosomal, 18S / isolation & purification*
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Republic of Korea
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Sequence Analysis, DNA
Substances
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DNA Primers
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DNA, Helminth
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RNA, Ribosomal, 18S
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DNA Restriction Enzymes