Evaluation of the Cepheid Xpert GBS assay for rapid detection of group B Streptococci in amniotic fluids from pregnant women with premature rupture of membranes

J Clin Microbiol. 2013 Apr;51(4):1305-6. doi: 10.1128/JCM.03356-12. Epub 2013 Feb 6.

Abstract

The Xpert GBS real-time PCR assay for the detection of group B streptococci (GBS) in antepartum screening samples was evaluated on amniotic fluid samples collected from 139 women with premature rupture of membrane at term. When any intrapartum positive result from the Xpert GBS or culture was considered a true positive, the sensitivities of the Xpert GBS and culture were 92.3% and 84.6%, respectively. This assay could enhance exact identification of candidates for intrapartum antibiotic prophylaxis.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Bacteriological Techniques / methods*
  • Female
  • Fetal Membranes, Premature Rupture*
  • Humans
  • Molecular Diagnostic Techniques / methods*
  • Pregnancy
  • Pregnancy Complications, Infectious / diagnosis*
  • Prospective Studies
  • Real-Time Polymerase Chain Reaction / methods*
  • Streptococcal Infections / diagnosis*
  • Streptococcus agalactiae / genetics
  • Streptococcus agalactiae / growth & development
  • Streptococcus agalactiae / isolation & purification*