Abstract
The prototypical death receptor Fas (also known as CD95 or Apo-1) plays an essential role in the maintenance of lymphocyte homeostasis. Propagation of cell death through Fas relies on the formation of a multiprotein complex at the receptor level known as the death-inducing signaling complex (DISC). Here, we describe an immunoprecipitation-based protocol to study DISC assembly in activated human T lymphocytes. This procedure is a useful tool to visualize proteins associated with Fas.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Cell Culture Techniques
-
Collodion / chemistry
-
Death Domain Receptor Signaling Adaptor Proteins / metabolism*
-
Electrophoresis, Polyacrylamide Gel
-
Humans
-
Immunoblotting
-
Immunoprecipitation / methods*
-
Lymphocyte Activation
-
Membranes, Artificial
-
Molecular Imaging / methods*
-
T-Lymphocytes / cytology
-
T-Lymphocytes / immunology
-
T-Lymphocytes / metabolism
-
fas Receptor / metabolism*
Substances
-
Death Domain Receptor Signaling Adaptor Proteins
-
Membranes, Artificial
-
fas Receptor
-
Collodion