Synchrotron X-ray fluorescence studies of a bromine-labelled cyclic RGD peptide interacting with individual tumor cells

J Synchrotron Radiat. 2013 Mar;20(Pt 2):226-33. doi: 10.1107/S0909049513001647. Epub 2013 Feb 7.

Abstract

The first example of synchrotron X-ray fluorescence imaging of cultured mammalian cells in cyclic peptide research is reported. The study reports the first quantitative analysis of the incorporation of a bromine-labelled cyclic RGD peptide and its effects on the biodistribution of endogenous elements (for example, K and Cl) within individual tumor cells.

Keywords: RGD peptide; XRF; microprobe; tumor cells.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Bromine
  • Humans
  • Lung Neoplasms / metabolism*
  • Melanoma, Experimental / metabolism*
  • Mice
  • Neovascularization, Pathologic / prevention & control
  • Oligopeptides / chemistry
  • Oligopeptides / metabolism
  • Peptides, Cyclic / chemistry*
  • Peptides, Cyclic / pharmacokinetics*
  • Spectrometry, X-Ray Emission / methods
  • Tumor Cells, Cultured

Substances

  • Oligopeptides
  • Peptides, Cyclic
  • cyclic arginine-glycine-aspartic acid peptide
  • arginyl-glycyl-aspartic acid
  • Bromine