Function of chromatin structure and dynamics in DNA damage, repair and misrepair: γ-rays and protons in action

Lucie Ježková; Martin Falk; Iva Falková; Marie Davídková; Alena Bačíková; Lenka Štefančíková; Jana Vachelová; Anna Michaelidesová; Emilie Lukášová; Alla Boreyko; Evgeny Krasavin; Stanislav Kozubek

doi:10.1016/j.apradiso.2013.01.022

Function of chromatin structure and dynamics in DNA damage, repair and misrepair: γ-rays and protons in action

Appl Radiat Isot. 2014 Jan:83 Pt B:128-36. doi: 10.1016/j.apradiso.2013.01.022. Epub 2013 Jan 20.

Authors

Lucie Ježková¹, Martin Falk, Iva Falková, Marie Davídková, Alena Bačíková, Lenka Štefančíková, Jana Vachelová, Anna Michaelidesová, Emilie Lukášová, Alla Boreyko, Evgeny Krasavin, Stanislav Kozubek

Affiliation

¹ Institute of Biophysics Brno, Academy of Sciences of the Czech Republic, Brno, Czech Republic; Joint Institute for Nuclear Research, Dubna, Moscow Region, Russia; Institute of Chemical Technology Prague, Prague, Czech Republic.

PMID: 23415104
DOI: 10.1016/j.apradiso.2013.01.022

Abstract

According to their physical characteristics, protons and ion beams promise a revolution in cancer radiotherapy. Curing protocols however reflect rather the empirical knowledge than experimental data on DNA repair. This especially holds for the spatio-temporal organization of repair processes in the context of higher-order chromatin structure-the problematics addressed in this work. The consequences for the mechanism of chromosomal translocations are compared for gamma rays and proton beams.

Keywords: DNA double-strand breaks (DSBs); Formation of chromosomal translocations; Gamma rays and proton beams; Higher-order chromatin structure and DSB repair; γH2AX foci.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Nucleus / radiation effects
Cells, Cultured
Chromatin / chemistry*
DNA Damage*
DNA Repair*
Gamma Rays*
Humans
Microscopy
Protons*
Translocation, Genetic

Substances

Chromatin
Protons