Simultaneous determination of cytosine arabinoside and its metabolite, uracil arabinoside, in human plasma using hydrophilic interaction liquid chromatography with UV detection

Biomed Chromatogr. 2013 Jul;27(7):818-20. doi: 10.1002/bmc.2876. Epub 2013 Feb 19.

Abstract

A practical high-performance liquid chromatography using a Cosmosil HILIC column and UV detection was developed for determining the concentrations of cytosine arabinoside (Ara-C) and uracil arabinoside (Ara-U), which is a major metabolite of Ara-C, in human plasma. This method was used to determine the plasma concentrations of Ara-C and Ara-U in a patient treated with high-dose Ara-C therapy for end-stage renal failure.

MeSH terms

  • Arabinofuranosyluracil / blood*
  • Arabinofuranosyluracil / chemistry
  • Chromatography, High Pressure Liquid / methods*
  • Cytarabine / blood*
  • Cytarabine / chemistry
  • Humans
  • Hydrophobic and Hydrophilic Interactions
  • Limit of Detection
  • Linear Models
  • Male
  • Middle Aged
  • Reproducibility of Results
  • Spectrophotometry, Ultraviolet

Substances

  • Cytarabine
  • Arabinofuranosyluracil