Fluorescence intensity- and lifetime-based glucose sensing using glucose/galactose-binding protein

J Diabetes Sci Technol. 2013 Jan 1;7(1):62-71. doi: 10.1177/193229681300700108.

Abstract

We review progress in our laboratories toward developing in vivo glucose sensors for diabetes that are based on fluorescence labeling of glucose/galactose-binding protein. Measurement strategies have included both monitoring glucose-induced changes in fluorescence resonance energy transfer and labeling with the environmentally sensitive fluorophore, badan. Measuring fluorescence lifetime rather than intensity has particular potential advantages for in vivo sensing. A prototype fiber-optic-based glucose sensor using this technology is being tested.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Biosensing Techniques / instrumentation*
  • Biosensing Techniques / methods
  • Blood Glucose / analysis*
  • Blood Glucose Self-Monitoring / instrumentation*
  • Blood Glucose Self-Monitoring / methods*
  • Calcium-Binding Proteins
  • Diabetes Mellitus / blood*
  • Fluorescence
  • Fluorescence Resonance Energy Transfer
  • Glucose
  • Humans
  • Monosaccharide Transport Proteins
  • Periplasmic Binding Proteins

Substances

  • Blood Glucose
  • Calcium-Binding Proteins
  • Monosaccharide Transport Proteins
  • Periplasmic Binding Proteins
  • galactose-binding protein
  • Glucose