Abstract
Twenty-four oleanane-type triterpenoid saponins were isolated from a methanol extract of the Pulsatilla koreana roots. Their structures were elucidated by comparing spectroscopic data to published values. Among them, compounds 8-12 and 20-24 significantly diminished the proliferation of HL-60 human promyelocytic leukemia cells with IC50 values from 0.3 to 4.2 μM, whereas compounds 7 and 19 showed moderate cytotoxic activity. Furthermore, apoptotic characteristics such as chromatin condensation and increase in the population of sub-G1 hypodiploid cells were observed after the HL-60 cells were treated with these compounds.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Antineoplastic Agents, Phytogenic / chemistry
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Antineoplastic Agents, Phytogenic / isolation & purification
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Antineoplastic Agents, Phytogenic / pharmacology
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Apoptosis / drug effects
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HL-60 Cells
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Humans
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Inhibitory Concentration 50
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Leukemia, Promyelocytic, Acute / drug therapy*
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Leukemia, Promyelocytic, Acute / pathology
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Oleanolic Acid / chemistry
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Oleanolic Acid / isolation & purification
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Oleanolic Acid / pharmacology*
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Plant Extracts / pharmacology
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Plant Roots
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Pulsatilla / chemistry*
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Saponins / chemistry
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Saponins / isolation & purification
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Saponins / pharmacology*
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Spectrum Analysis
Substances
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Antineoplastic Agents, Phytogenic
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Plant Extracts
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Saponins
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Oleanolic Acid