Structure of gene coding for rat group II phospholipase A2

Biochem Biophys Res Commun. 1990 May 16;168(3):1059-65. doi: 10.1016/0006-291x(90)91137-h.

Abstract

The gene coding for rat group II phospholipase A2 was isolated from a rat genomic library by using the cDNA for rat platelet phospholipase A2 as a hybridization probe. The rat group II phospholipase A2 gene spanned about 3.5 kilobase pairs and consisted of five exons. Southern blot analysis revealed that a single copy of this gene exists in the rat haploid genome. A TATA-like sequence and two AP-2 binding site-like loci were found upstream from the tentatively identified transcription initiation site.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Southern
  • DNA / isolation & purification
  • Exons
  • Gene Expression
  • Gene Library
  • Molecular Sequence Data
  • Phospholipases / genetics*
  • Phospholipases A / genetics*
  • Phospholipases A2
  • Rats
  • Restriction Mapping
  • Spleen / enzymology

Substances

  • DNA
  • Phospholipases
  • Phospholipases A
  • Phospholipases A2

Associated data

  • GENBANK/M37127