Angiopoietin-2 is associated with albuminuria and microinflammation in chronic kidney disease

PLoS One. 2013;8(3):e54668. doi: 10.1371/journal.pone.0054668. Epub 2013 Mar 1.

Abstract

Although cardiovascular disease (CVD) is the leading cause of mortality in patients with chronic kidney disease (CKD), the pathophysiology is not thoroughly understood. Given that elevated albuminuria or circulating angiopoietin-2 associates with CVD and mortality in CKD patients, we were intrigued by the relationship between albuminuria and angiopoietin-2. A total of 416 patients with CKD stages 3 to 5 were stratified by urine albumin-creatinine ratio as normoalbuminuria (<30 mg/g), microalbuminuria (30-300 mg/g), or macroalbuminuria (>300 mg/g). The levels of plasma angiopoietin-2 and vascular endothelial growth factor (VEGF) increased, and soluble Tie-2 decreased in the subgroups of albuminuria; whereas angiopoietin-1 did not change. Linear regression showed a positive correlation between urine albumin-creatinine ratio (ACR) and plasma angiopoietin-2 (correlation coefficient r = 0.301, 95% confidence interval 0.211-0.386, P<0.0001), but not between ACR and VEGF or soluble Tie-2. Multivariate linear regression analysis showed that plasma angiopoietin-2 was independently associated with ACR (P = 0.025). Furthermore, plasma angiopoietin-2 was positively correlated with high sensitive C-reactive protein (r = 0.114, 95% confidence interval 0.018-0.208, P = 0.020). In conclusion, plasma angiopoietin-2 was associated with albuminuria and markers of systemic microinflammation in CKD patients. Although previous evidence has shown that angiopoietin-2 destabilizes vasculature and induces inflammation in different scenarios, further study will be required to delineate the role of angiopoietin-2 in albuminuria and microinflammation in CKD patients.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Albuminuria / blood*
  • Albuminuria / complications
  • Albuminuria / physiopathology
  • Angiopoietin-1 / blood
  • Angiopoietin-2 / blood*
  • Biomarkers / blood
  • C-Reactive Protein / metabolism
  • Cardiovascular Diseases / blood*
  • Cardiovascular Diseases / complications
  • Cardiovascular Diseases / physiopathology
  • Creatinine / urine
  • Female
  • Glomerular Filtration Rate
  • Humans
  • Inflammation / blood
  • Male
  • Middle Aged
  • Multivariate Analysis
  • Renal Insufficiency, Chronic / blood*
  • Renal Insufficiency, Chronic / complications
  • Renal Insufficiency, Chronic / physiopathology
  • Vascular Endothelial Growth Factor A / blood

Substances

  • Angiopoietin-1
  • Angiopoietin-2
  • Biomarkers
  • VEGFA protein, human
  • Vascular Endothelial Growth Factor A
  • C-Reactive Protein
  • Creatinine

Grants and funding

This study was supported by the National Science Council (99-2628-B-002-013, S.L.L., 101-2321-B-002-060, S.L.L.) (http://web1.nsc.gov.tw/), and Mrs. Hsiu-Chin Lee Kidney Research Foundation. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.