Abstract
We incorporated a previously identified mutation that reduces the fidelity of the DNA polymerase into a human adenovirus vector. Using this mutator vector, we demonstrate rapid selection of resistance to a neutralizing anti-hexon monoclonal antibody due to a G434D mutation in hexon that precludes antibody binding. Since mutator adenoviruses can accumulate compound mutations that are unattainable using traditional random mutagenesis techniques, this approach will be valuable to the study of antivirals and host factor interactions.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenoviruses, Human / genetics*
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Adenoviruses, Human / growth & development
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Adenoviruses, Human / immunology*
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Amino Acid Substitution
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Antibodies, Neutralizing / immunology*
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Antibodies, Viral / immunology*
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Capsid Proteins / genetics
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Capsid Proteins / immunology
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Cell Line
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Directed Molecular Evolution*
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Genetics, Microbial / methods
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Humans
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Mutation, Missense
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Viral Load
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Virology / methods
Substances
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Antibodies, Neutralizing
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Antibodies, Viral
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Capsid Proteins
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hexon capsid protein, Adenovirus