Panax ginseng polysaccharide suppresses metastasis via modulating Twist expression in gastric cancer

Jian-Ping Cai; Yang-Jiazi Wu; Cong Li; Mei-Yan Feng; Qing-Tao Shi; Rui Li; Zhen-Yu Wang; Jing-Shu Geng

doi:10.1016/j.ijbiomac.2013.03.010

Panax ginseng polysaccharide suppresses metastasis via modulating Twist expression in gastric cancer

Int J Biol Macromol. 2013 Jun:57:22-5. doi: 10.1016/j.ijbiomac.2013.03.010. Epub 2013 Mar 13.

Authors

Jian-Ping Cai¹, Yang-Jiazi Wu, Cong Li, Mei-Yan Feng, Qing-Tao Shi, Rui Li, Zhen-Yu Wang, Jing-Shu Geng

Affiliation

¹ Department of Pathology, The Third Affiliated (Tumor) Hospital, Harbin Medical University, Harbin 150040, China.

PMID: 23500436
DOI: 10.1016/j.ijbiomac.2013.03.010

Abstract

It was previously reported that an antitumor polysaccharide (PGPW1) was isolated from the root of Panax ginseng. To extend our study, we investigated here the anti-invasive and metastatic effects of PGPW1 on human gastric cancer cell line HGC-27 and tried to determine its possible mechanism of action. Both scratch wound-healing and Transwell assay identified that PGPW1 dose-dependently inhibited migration and invasiveness of HGC-27 cells. Furthermore, results of western blot showed that protein levels of Twist and AKR1C2 were inhibited by PGPW1, whereas an increase of NF1 was observed. Moreover, down-regulation of Twist expression by PGPW1 blocked epithelial-mesenchymal transition (EMT), characterized by a gain of epithelial cell markers, E-cadherin, and loss of the mesenchymal markers, vimentin and N-cadherin, at protein levels. Collectively, we confirmed that PGPW1 decreased migration and invasion of HGC-27 cells by regulation of Twist, AKR1C2, NF1, E-cadherin, vimentin and N-cadherin expression. In conclusion, PGPW1 may serve as a powerful chemopreventive agent against gastric cancer metastasis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antigens, CD / biosynthesis
Antigens, CD / genetics
Cadherins / biosynthesis
Cadherins / genetics
Cell Line, Tumor
Down-Regulation / drug effects
Down-Regulation / genetics
Epithelial-Mesenchymal Transition / drug effects
Epithelial-Mesenchymal Transition / genetics
Gene Expression Regulation, Neoplastic / drug effects*
Gene Expression Regulation, Neoplastic / genetics
Humans
Hydroxysteroid Dehydrogenases / biosynthesis
Hydroxysteroid Dehydrogenases / genetics
Neoplasm Metastasis
Neurofibromin 1 / biosynthesis
Neurofibromin 1 / genetics
Nuclear Proteins / biosynthesis*
Nuclear Proteins / genetics
Panax / chemistry*
Polysaccharides / chemistry
Polysaccharides / pharmacology*
Stomach Neoplasms / genetics
Stomach Neoplasms / metabolism*
Stomach Neoplasms / pathology
Twist-Related Protein 1 / biosynthesis*
Twist-Related Protein 1 / genetics
Vimentin / biosynthesis
Vimentin / genetics

Substances

Antigens, CD
CDH2 protein, human
Cadherins
Neurofibromin 1
Nuclear Proteins
Polysaccharides
TWIST1 protein, human
Twist-Related Protein 1
Vimentin
Hydroxysteroid Dehydrogenases
AKR1C2 protein, human