Thermal and optical characterization of micro-LED probes for in vivo optogenetic neural stimulation

Opt Lett. 2013 Mar 15;38(6):992-4. doi: 10.1364/OL.38.000992.

Abstract

Within optogenetics there is a need for compact light sources that are capable of delivering light with excellent spatial, temporal, and spectral resolution to deep brain structures. Here, we demonstrate a custom GaN-based LED probe for such applications and the electrical, optical, and thermal properties are analyzed. The output power density and emission spectrum were found to be suitable for stimulating channelrhodopsin-2, one of the most common light-sensitive proteins currently used in optogenetics. The LED device produced high light intensities, far in excess of those required to stimulate the light-sensitive proteins within the neurons. Thermal performance was also investigated, illustrating that a broad range of operating regimes in pulsed mode are accessible while keeping a minimum increase in temperature for the brain (0.5°C). This type of custom device represents a significant step forward for the optogenetics community, allowing multiple bright excitation sites along the length of a minimally invasive neural probe.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Brain / cytology
  • Electricity
  • Gallium
  • Microtechnology / instrumentation*
  • Neurons / cytology*
  • Optical Devices*
  • Optogenetics / instrumentation*
  • Temperature*

Substances

  • gallium nitride
  • Gallium