PCB 77 dechlorination products modulate pro-inflammatory events in vascular endothelial cells

Environ Sci Pollut Res Int. 2014 May;21(10):6354-64. doi: 10.1007/s11356-013-1591-3. Epub 2013 Mar 16.

Abstract

Persistent organic pollutants such as polychlorinated biphenyls (PCBs) are associated with detrimental health outcomes including cardiovascular diseases. Remediation of these compounds is a critical component of environmental policy. Although remediation efforts aim to completely remove toxicants, little is known about the effects of potential remediation byproducts. We previously published that Fe/Pd nanoparticles effectively dechlorinate PCB 77 to biphenyl, thus eliminating PCB-induced endothelial dysfunction using primary vascular endothelial cells. Herein, we analyzed the toxic effects of PCB congener mixtures (representative mixtures of commercial PCBs based on previous dechlorination data) produced at multiple time points during the dechlorination of PCB 77 to biphenyl. Compared with pure PCB 77, exposing endothelial cells to lower chlorinated PCB byproducts led to improved cellular viability, decreased superoxide production, and decreased nuclear factor kappa B activation based on duration of remediation. Presence of the parent compound, PCB 77, led to significant increases in mRNA and protein inflammatory marker expression. These data implicate that PCB dechlorination reduces biological toxicity to vascular endothelial cells.

Publication types

  • Research Support, American Recovery and Reinvestment Act
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Endothelial Cells
  • Gene Expression / drug effects
  • Hazardous Substances / toxicity*
  • Polychlorinated Biphenyls / toxicity*

Substances

  • Hazardous Substances
  • Polychlorinated Biphenyls
  • 3,4,3',4'-tetrachlorobiphenyl