High-yield production of dihydrogen from xylose by using a synthetic enzyme cascade in a cell-free system

Julia S Martín del Campo; Joseph Rollin; Suwan Myung; You Chun; Sanjeev Chandrayan; Rodrigo Patiño; Michael W W Adams; Y-H Percival Zhang

doi:10.1002/anie.201300766

High-yield production of dihydrogen from xylose by using a synthetic enzyme cascade in a cell-free system

Angew Chem Int Ed Engl. 2013 Apr 22;52(17):4587-90. doi: 10.1002/anie.201300766. Epub 2013 Mar 19.

Authors

Julia S Martín del Campo¹, Joseph Rollin, Suwan Myung, You Chun, Sanjeev Chandrayan, Rodrigo Patiño, Michael W W Adams, Y-H Percival Zhang

Affiliation

¹ Biological Systems Engineering Department, Virginia Tech, Blacksburg, VA 24061, USA.

PMID: 23512726
DOI: 10.1002/anie.201300766

Abstract

Let enzymes work: H2 was produced from xylose and water in one reactor containing 13 enzymes (red). By using a novel polyphosphate xylulokinase (XK), xylose was converted into H2 and CO2 with approaching 100 % of the theoretical yield. The findings suggest that cell-free biosystems could produce H2 from biomass xylose at low cost. Xu5P = xylulose 5-phosphate, G6P = glucose 6-phosphate.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Acid Anhydride Hydrolases / chemistry
Aldose-Ketose Isomerases / chemistry
Biocatalysis
Cell-Free System / chemistry*
Cell-Free System / enzymology
Enzymes, Immobilized / chemistry*
Hydrogen / chemistry*
Hydrogenase / chemistry
Xylose / chemistry*

Substances

Enzymes, Immobilized
Hydrogen
Xylose
Hydrogenase
Acid Anhydride Hydrolases
endopolyphosphatase
Aldose-Ketose Isomerases
xylose isomerase